After decoding human genome and several model animal genomes, the study protein encoding ORF expression in vitro and in vivo, no-protein encoding sequence such as micro RNAs and their targets and net-work of biomacro molecules such as protein-protein and DNA-protein interactions requires advanced and innovative technologies. Our lab is focusing on development of methods and technologies related to promoters, fusion tags, report molecules, DNA polymerase chain reaction (PCR), expression vectors and DNA transferring platform for enhancing recombinant protein expression in cell host and cell free protein production system, for easy purification and for efficient immobilization, cellular localization and high throughput detection.